Research Tech III - Computational Immunology at CHOP/Dr.Li Lab
SHIFT:
Any (United States of America)Seeking Breakthrough MakersChildren's Hospital of Philadelphia (CHOP) offers countless ways to change lives.
Our diverse community of more than 20,000 Breakthrough Makers will inspire you to pursue passions, develop expertise, and drive innovation.
At CHOP, your experience is valued; your voice is heard; and your contributions make a difference for patients and families.
Join us as we build on our promise to advance pediatric care-and your career.
CHOP's Commitment to Diversity, Equity, and InclusionCHOP is committed to building an inclusive culture where employees feel a sense of belonging, connection, and community within their workplace.
We are a team dedicated to fostering an environment that allows for all to be their authentic selves.
We are focused on attracting, cultivating, and retaining diverse talent who can help us deliver on our mission to be a world leader in the advancement of healthcare for children.
We strongly encourage all candidates of diverse backgrounds and lived experiences to apply.
A Brief OverviewLi lab of Computational Immunology at CHOP is looking for a self-disciplined, responsible full-time lab technician to manage daily lab activities, participate in basic molecular and cell experiments, and carry out other duties as assigned.
An ideal applicant is expected to be proficient in some or all of the following aspects:
1.
Immunology:
Cell culture (experience of primary T cell culture is a plus); flow sorting; EliSPOT; T cell killing assay; experience with single-chain trimer is considered a big plus.
2.
Cellular and molecular techniques:
Mammalian cell culture; PCR and vector construction; Genomic DNA isolation; RNA extraction; Reverse transcription; Real-time PCR; Cell transfection; Recombinant adenovirus package; Amplification and purification; FACS analysis; Immunohistochemistry and immunofluorescent assay.
3.
Genomics:
scRNA-seq library construction; ATAC-seq library construction; CRISPR KO4.
Excellent communication skills including both speaking and writing.
The applicant is expected to hold a degree in biology/medicine or other equivalent areas.
Individuals with at least 2 years of lab management experiences are preferred.
The PI is an incoming Associate Professor at Department of Pathology and Laboratory Medicine of Penn/CHOP.
The lab is supported by multiple NIH grants as well as a internal funding.
What you will do Performs research procedures on experimental samples with minimal supervision Formulates and performs complex lab experiments and analyzes & reports the data Performs tasks that require knowledge of advanced techniques and equipment Performs advanced calculations and maintains appropriate log and lab notebooks Assists with planning specific research protocols and procedures Assists in development & troubleshooting of new techniques and ideas May be required to modify scientific methods and protocols (in conjunction with PI) Checks others results for completeness and accuracy May be responsible for training and mentoring new staff Assists in the preparing of grants journal articles, poster and oral presentations May perform routine and non-routine tests / experiments as needed Education Qualifications Associate's Degree Required Bachelor's Degree Preferred Experience Qualifications At least one (1) year laboratory experience Required At least two (2) years laboratory experience Preferred To carry out its mission, CHOP is committed to supporting the health of our patients, families, workforce, and global community.
As a condition of employment, CHOP employees who work in patient care buildings or who have patient facing responsibilities must be fully vaccinated against COVID-19 and receive an annual influenza vaccine.
Learn more.
Employees may request exemptions for valid religious and medical reasons.
Start dates may be delayed until candidates are immunized or exemption requests are reviewed.
EEO / VEVRAA Federal Contractor Tobacco Statement Recommended Skills Biology Calculations Coaching And Mentoring Communication Construction Crispr Estimated Salary: $20 to $28 per hour based on qualifications.
Any (United States of America)Seeking Breakthrough MakersChildren's Hospital of Philadelphia (CHOP) offers countless ways to change lives.
Our diverse community of more than 20,000 Breakthrough Makers will inspire you to pursue passions, develop expertise, and drive innovation.
At CHOP, your experience is valued; your voice is heard; and your contributions make a difference for patients and families.
Join us as we build on our promise to advance pediatric care-and your career.
CHOP's Commitment to Diversity, Equity, and InclusionCHOP is committed to building an inclusive culture where employees feel a sense of belonging, connection, and community within their workplace.
We are a team dedicated to fostering an environment that allows for all to be their authentic selves.
We are focused on attracting, cultivating, and retaining diverse talent who can help us deliver on our mission to be a world leader in the advancement of healthcare for children.
We strongly encourage all candidates of diverse backgrounds and lived experiences to apply.
A Brief OverviewLi lab of Computational Immunology at CHOP is looking for a self-disciplined, responsible full-time lab technician to manage daily lab activities, participate in basic molecular and cell experiments, and carry out other duties as assigned.
An ideal applicant is expected to be proficient in some or all of the following aspects:
1.
Immunology:
Cell culture (experience of primary T cell culture is a plus); flow sorting; EliSPOT; T cell killing assay; experience with single-chain trimer is considered a big plus.
2.
Cellular and molecular techniques:
Mammalian cell culture; PCR and vector construction; Genomic DNA isolation; RNA extraction; Reverse transcription; Real-time PCR; Cell transfection; Recombinant adenovirus package; Amplification and purification; FACS analysis; Immunohistochemistry and immunofluorescent assay.
3.
Genomics:
scRNA-seq library construction; ATAC-seq library construction; CRISPR KO4.
Excellent communication skills including both speaking and writing.
The applicant is expected to hold a degree in biology/medicine or other equivalent areas.
Individuals with at least 2 years of lab management experiences are preferred.
The PI is an incoming Associate Professor at Department of Pathology and Laboratory Medicine of Penn/CHOP.
The lab is supported by multiple NIH grants as well as a internal funding.
What you will do Performs research procedures on experimental samples with minimal supervision Formulates and performs complex lab experiments and analyzes & reports the data Performs tasks that require knowledge of advanced techniques and equipment Performs advanced calculations and maintains appropriate log and lab notebooks Assists with planning specific research protocols and procedures Assists in development & troubleshooting of new techniques and ideas May be required to modify scientific methods and protocols (in conjunction with PI) Checks others results for completeness and accuracy May be responsible for training and mentoring new staff Assists in the preparing of grants journal articles, poster and oral presentations May perform routine and non-routine tests / experiments as needed Education Qualifications Associate's Degree Required Bachelor's Degree Preferred Experience Qualifications At least one (1) year laboratory experience Required At least two (2) years laboratory experience Preferred To carry out its mission, CHOP is committed to supporting the health of our patients, families, workforce, and global community.
As a condition of employment, CHOP employees who work in patient care buildings or who have patient facing responsibilities must be fully vaccinated against COVID-19 and receive an annual influenza vaccine.
Learn more.
Employees may request exemptions for valid religious and medical reasons.
Start dates may be delayed until candidates are immunized or exemption requests are reviewed.
EEO / VEVRAA Federal Contractor Tobacco Statement Recommended Skills Biology Calculations Coaching And Mentoring Communication Construction Crispr Estimated Salary: $20 to $28 per hour based on qualifications.
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