Research Tech II-Oncology/ Dr. Wolpaw/ Dr. Bosse lab
SHIFT:
Day (United States of America)Seeking Breakthrough MakersChildren's Hospital of Philadelphia (CHOP) offers countless ways to change lives.
Our diverse community of more than 20,000 Breakthrough Makers will inspire you to pursue passions, develop expertise, and drive innovation.
At CHOP, your experience is valued; your voice is heard; and your contributions make a difference for patients and families.
Join us as we build on our promise to advance pediatric care-and your career.
CHOP's Commitment to Diversity, Equity, and InclusionCHOP is committed to building an inclusive culture where employees feel a sense of belonging, connection, and community within their workplace.
We are a team dedicated to fostering an environment that allows for all to be their authentic selves.
We are focused on attracting, cultivating, and retaining diverse talent who can help us deliver on our mission to be a world leader in the advancement of healthcare for children.
We strongly encourage all candidates of diverse backgrounds and lived experiences to apply.
A Brief OverviewThe Research Technician II will have a BS or BA degree in the biological or chemical sciences or another scientific discipline and will conduct translational pediatric cancer and immuno-oncology research under the direction/guidance of Drs.
Kris Bosse and Adam Wolpaw in collaboration with the St.
Baldricks-Stand Up To Cancer Immunogenomics Team (https:
//standuptocancer.
org/research/research-portfolio/dream-teams/high-risk-pediatric-cancers-dream-team/).
The Bosse and Wolpaw labs are focused the development of novel immunotherapeutic approaches to treatment of childhood solid tumors, particularly neuroblastoma.
Dr.
Bosse's work centers on targeting tumor integral cell surfaces molecules (see Bosse et al.
, 2017 Cancer Cell) and using circulating tumor DNA (ctDNA) to define mechanisms of therapeutic resistance and disease progression (see Bosse et al.
, 2022 Cancer Discovery).
The Wolpaw laboratory is focused on understanding how lineage heterogeneity impacts inflammatory sensing and immunotherapy response (see Wolpaw et al.
, 2022 PNAS).
The research technician will join these growing translational research teams and will be co-mentored by Drs.
Bosse and Wolpaw and work in collaboration with several other laboratories at CHOP/Penn.
Ongoing projects in the Bosse laboratory include translating current immunotherapies in the lab across pediatric cancers, new immunotherapeutic target discovery in neuroblastoma and other pediatric cancers, chimeric antigen receptor T cell therapy and antibody-drug conjugate development, defining mechanisms of tumor cell intrinsic and extrinsic immune escape using both immunodeficient and immunocompetent murine models, deciphering the key biologic functions of prioritized immunotherapeutic targets and using serial neuroblastoma preclinical models to define mechanisms of therapeutic resistance.
Ongoing projects in the Wolpaw Lab include using single-cell RNA sequencing to define how neuroblastoma lineage heterogeneity impacts tumor-immune interactions in mouse models of neuroblastoma, conducting CRISPR-Cas9 screens to uncover the transcriptional regulation of innate immune sensing pathways, and characterizing how activation of inflammatory signaling in neuroblastoma cells impact immune surveillance and immunotherapy responses.
Diverse molecular biology, genomic, and in vitro and in vivo laboratory techniques will be employed, and ideally the applicant will be experienced with or have a desire to learn in vivo techniques utilizing several murine models of neuroblastoma.
The ideal candidate will (1) Work independently and be self-directed with guidance from the principal investigators, (2) Develop new experimental procedures and hypotheses for further investigation and will help the principle investigators publish and present research results, (3) Assist with the management of laboratory operations, (4) Collaborate and interact with other researchers within the laboratories, institution and at other institutions, (5) Consistently exercise discretion and judgment in execution, interpretation of experiments, and of data derived, and (6) Have some laboratory experience with techniques such as cell culture, RNA purification, PCR, cloning, Western blotting, in vitro functional assays, flow cytometry, and some experience working with mouse models of cancer.
Ideally looking for someone to commit to our laboratories for several years and at least a 2-year commitment is required.
Will have multiple opportunities to present work at national and international meetings and will be involved in writing manuscripts.
Laboratory environment includes a diverse range of scientists and trainees at all career levels, including research technicians doing a 2-3 year laboratory experience prior to applying to professional (e.
g.
Graduate or Medical) school.
What you will do Performs routine tests under the direction of principal investigator or supervisor Performs research procedures on experimental samples as directed Records and checks results for completeness and accuracy Performs calculations and maintains appropriate log and lab notebooks Performs tasks that require familiarity with fundamental techniques and equipment Assists with planning specific research protocols and procedures Maintains office, lab area, reagents, supplies and equipment Cleans and performs routine maintenance on lab equipment Coordinates schedules of tests & procedures Attends courses, seminars, and lab meetings to expand knowledge of research and laboratory techniques Records results, organizes data and maintains lab notebook in an organized manner Conducts literary searches as directed Education Qualifications Associate's Degree Required Bachelor's Degree Preferred Experience Qualifications At least six (6) months laboratory experience Required At least one (1) year laboratory experience Preferred Related internship experience Preferred To carry out its mission, CHOP is committed to supporting the health of our patients, families, workforce, and global community.
As a condition of employment, CHOP employees who work in patient care buildings or who have patient facing responsibilities must be fully vaccinated against COVID-19 and receive an annual influenza vaccine.
Learn more.
Employees may request exemptions for valid religious and medical reasons.
Start dates may be delayed until candidates are immunized or exemption requests are reviewed.
EEO / VEVRAA Federal Contractor Tobacco Statement Recommended Skills Antibodies Biology Calculations Cell Cultures Cell Therapy Cloning (Biology) Estimated Salary: $20 to $28 per hour based on qualifications.
Day (United States of America)Seeking Breakthrough MakersChildren's Hospital of Philadelphia (CHOP) offers countless ways to change lives.
Our diverse community of more than 20,000 Breakthrough Makers will inspire you to pursue passions, develop expertise, and drive innovation.
At CHOP, your experience is valued; your voice is heard; and your contributions make a difference for patients and families.
Join us as we build on our promise to advance pediatric care-and your career.
CHOP's Commitment to Diversity, Equity, and InclusionCHOP is committed to building an inclusive culture where employees feel a sense of belonging, connection, and community within their workplace.
We are a team dedicated to fostering an environment that allows for all to be their authentic selves.
We are focused on attracting, cultivating, and retaining diverse talent who can help us deliver on our mission to be a world leader in the advancement of healthcare for children.
We strongly encourage all candidates of diverse backgrounds and lived experiences to apply.
A Brief OverviewThe Research Technician II will have a BS or BA degree in the biological or chemical sciences or another scientific discipline and will conduct translational pediatric cancer and immuno-oncology research under the direction/guidance of Drs.
Kris Bosse and Adam Wolpaw in collaboration with the St.
Baldricks-Stand Up To Cancer Immunogenomics Team (https:
//standuptocancer.
org/research/research-portfolio/dream-teams/high-risk-pediatric-cancers-dream-team/).
The Bosse and Wolpaw labs are focused the development of novel immunotherapeutic approaches to treatment of childhood solid tumors, particularly neuroblastoma.
Dr.
Bosse's work centers on targeting tumor integral cell surfaces molecules (see Bosse et al.
, 2017 Cancer Cell) and using circulating tumor DNA (ctDNA) to define mechanisms of therapeutic resistance and disease progression (see Bosse et al.
, 2022 Cancer Discovery).
The Wolpaw laboratory is focused on understanding how lineage heterogeneity impacts inflammatory sensing and immunotherapy response (see Wolpaw et al.
, 2022 PNAS).
The research technician will join these growing translational research teams and will be co-mentored by Drs.
Bosse and Wolpaw and work in collaboration with several other laboratories at CHOP/Penn.
Ongoing projects in the Bosse laboratory include translating current immunotherapies in the lab across pediatric cancers, new immunotherapeutic target discovery in neuroblastoma and other pediatric cancers, chimeric antigen receptor T cell therapy and antibody-drug conjugate development, defining mechanisms of tumor cell intrinsic and extrinsic immune escape using both immunodeficient and immunocompetent murine models, deciphering the key biologic functions of prioritized immunotherapeutic targets and using serial neuroblastoma preclinical models to define mechanisms of therapeutic resistance.
Ongoing projects in the Wolpaw Lab include using single-cell RNA sequencing to define how neuroblastoma lineage heterogeneity impacts tumor-immune interactions in mouse models of neuroblastoma, conducting CRISPR-Cas9 screens to uncover the transcriptional regulation of innate immune sensing pathways, and characterizing how activation of inflammatory signaling in neuroblastoma cells impact immune surveillance and immunotherapy responses.
Diverse molecular biology, genomic, and in vitro and in vivo laboratory techniques will be employed, and ideally the applicant will be experienced with or have a desire to learn in vivo techniques utilizing several murine models of neuroblastoma.
The ideal candidate will (1) Work independently and be self-directed with guidance from the principal investigators, (2) Develop new experimental procedures and hypotheses for further investigation and will help the principle investigators publish and present research results, (3) Assist with the management of laboratory operations, (4) Collaborate and interact with other researchers within the laboratories, institution and at other institutions, (5) Consistently exercise discretion and judgment in execution, interpretation of experiments, and of data derived, and (6) Have some laboratory experience with techniques such as cell culture, RNA purification, PCR, cloning, Western blotting, in vitro functional assays, flow cytometry, and some experience working with mouse models of cancer.
Ideally looking for someone to commit to our laboratories for several years and at least a 2-year commitment is required.
Will have multiple opportunities to present work at national and international meetings and will be involved in writing manuscripts.
Laboratory environment includes a diverse range of scientists and trainees at all career levels, including research technicians doing a 2-3 year laboratory experience prior to applying to professional (e.
g.
Graduate or Medical) school.
What you will do Performs routine tests under the direction of principal investigator or supervisor Performs research procedures on experimental samples as directed Records and checks results for completeness and accuracy Performs calculations and maintains appropriate log and lab notebooks Performs tasks that require familiarity with fundamental techniques and equipment Assists with planning specific research protocols and procedures Maintains office, lab area, reagents, supplies and equipment Cleans and performs routine maintenance on lab equipment Coordinates schedules of tests & procedures Attends courses, seminars, and lab meetings to expand knowledge of research and laboratory techniques Records results, organizes data and maintains lab notebook in an organized manner Conducts literary searches as directed Education Qualifications Associate's Degree Required Bachelor's Degree Preferred Experience Qualifications At least six (6) months laboratory experience Required At least one (1) year laboratory experience Preferred Related internship experience Preferred To carry out its mission, CHOP is committed to supporting the health of our patients, families, workforce, and global community.
As a condition of employment, CHOP employees who work in patient care buildings or who have patient facing responsibilities must be fully vaccinated against COVID-19 and receive an annual influenza vaccine.
Learn more.
Employees may request exemptions for valid religious and medical reasons.
Start dates may be delayed until candidates are immunized or exemption requests are reviewed.
EEO / VEVRAA Federal Contractor Tobacco Statement Recommended Skills Antibodies Biology Calculations Cell Cultures Cell Therapy Cloning (Biology) Estimated Salary: $20 to $28 per hour based on qualifications.
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